Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vitro Antimicrobial Activity of Diacerein on 76 Isolates of Gram-Positive Cocci from Bacterial Keratitis Patients and In Vivo Study of Diacerein Eye Drops on Staphylococcus aureus Keratitis in Mice

doi: 10.1128/AAC.01874-18

Figure Lengend Snippet: MIC range, geometric mean, MIC mode, MIC 50 , and MIC 90 of diacerein and its metabolite rhein against 65 common clinical ocular Gram-positive bacterial isolates

Article Snippet: Two Staphylococcus aureus reference strains (ATCC 25923 and ATCC 29213; a kind gift from the Department of Clinical Laboratory of Zhengzhou Yihe Hospital) were used as quality controls for the disk diffusion method and dilution susceptibility tests. (ii) Determining the MIC of diacerein.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vitro Antimicrobial Activity of Diacerein on 76 Isolates of Gram-Positive Cocci from Bacterial Keratitis Patients and In Vivo Study of Diacerein Eye Drops on Staphylococcus aureus Keratitis in Mice

doi: 10.1128/AAC.01874-18

Figure Lengend Snippet: MICs of diacerein and rhein against 11 uncommon clinical ocular Gram-positive bacterial isolates

Article Snippet: Two Staphylococcus aureus reference strains (ATCC 25923 and ATCC 29213; a kind gift from the Department of Clinical Laboratory of Zhengzhou Yihe Hospital) were used as quality controls for the disk diffusion method and dilution susceptibility tests. (ii) Determining the MIC of diacerein.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vitro Antimicrobial Activity of Diacerein on 76 Isolates of Gram-Positive Cocci from Bacterial Keratitis Patients and In Vivo Study of Diacerein Eye Drops on Staphylococcus aureus Keratitis in Mice

doi: 10.1128/AAC.01874-18

Figure Lengend Snippet: Corneal clinical scores in Staphylococcus aureus-infected BALB/c mice treated with normal saline (NS), levofloxacin (LF), and diacerein (DA) eye drop administered with 2-h, 1-h, and 0.5-h intervals (DA 2 h, DA 1 h, and DA 0.5 h) for 12 h. Eyes treated with LF, DA 1 h, and DA 0.5 h had significantly lower scores than those in NS-treated eyes, as indicated by an asterisk (**, P < 0.01). Error bars indicate standard error of the mean (SEM).

Article Snippet: Two Staphylococcus aureus reference strains (ATCC 25923 and ATCC 29213; a kind gift from the Department of Clinical Laboratory of Zhengzhou Yihe Hospital) were used as quality controls for the disk diffusion method and dilution susceptibility tests. (ii) Determining the MIC of diacerein.

Techniques: Infection, Saline

Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vitro Antimicrobial Activity of Diacerein on 76 Isolates of Gram-Positive Cocci from Bacterial Keratitis Patients and In Vivo Study of Diacerein Eye Drops on Staphylococcus aureus Keratitis in Mice

doi: 10.1128/AAC.01874-18

Figure Lengend Snippet: Average CFU per cornea (log10) in Staphylococcus aureus-infected BALB/c mice treated with normal saline (NS), levofloxacin (LF), and diacerein (DA) eye drops administered with 2-h, 1-h, and 0.5-h intervals (DA 2 h, DA 1 h, and DA 0.5 h) for 12 h. Corneas treated with LF and DA 2 h, DA 1 h, and DA 0.5 h had significantly lower CFU than NS-treated corneas, as indicated by an asterisk (**, P < 0.01). LF-treated eyes produced a significant reduction in corneal bacteria compared with DA 2 h, DA 1 h, and DA 0.5 h (**, P < 0.01). DA 1 h- and DA 0.5 h-treated eyes produced a significant reduction in corneal bacteria compared with DA2 h (P < 0.01). The number of viable Staphylococcus aureus per cornea was quantified and expressed as base 10 logarithms ± standard deviations (indicated by error bars). Data are from eight independent samples.

Article Snippet: Two Staphylococcus aureus reference strains (ATCC 25923 and ATCC 29213; a kind gift from the Department of Clinical Laboratory of Zhengzhou Yihe Hospital) were used as quality controls for the disk diffusion method and dilution susceptibility tests. (ii) Determining the MIC of diacerein.

Techniques: Infection, Saline, Eye Drops, Produced, Bacteria

Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vitro Antimicrobial Activity of Diacerein on 76 Isolates of Gram-Positive Cocci from Bacterial Keratitis Patients and In Vivo Study of Diacerein Eye Drops on Staphylococcus aureus Keratitis in Mice

doi: 10.1128/AAC.01874-18

Figure Lengend Snippet: The typical corneal histopathology images of Staphylococcus aureus-infected BALB/c mice treated with normal saline (NS), diacerein with administered at 1-h interval (DA 1 h), and levofloxacin (LF) eye drops for 12 h. Sections were stained with hematoxylin and eosin and Gram. Treatment with NS (negative control, A) demonstrating focally severe PMN accumulation throughout cornea, severe edema, and mass Gram-positive cocci in the anterior stroma. Treatment with LF (positive control, B) demonstrated minimal PMN infiltration mostly in anterior stroma, mild edema, and small Gram-positive cocci in the anterior stroma; treatment with DA (C) demonstrated moderate PMN infiltration in the anterior and middle stroma, moderate edema, and medium bacterial load in the anterior stroma. The arrows indicate the endothelium. The triangle indicates Gram-positive Staphylococcus aureus (dark purple). Scale bars: H&E, 20 μm; Gram, 20 μm (left) and 10 μm (right).

Article Snippet: Two Staphylococcus aureus reference strains (ATCC 25923 and ATCC 29213; a kind gift from the Department of Clinical Laboratory of Zhengzhou Yihe Hospital) were used as quality controls for the disk diffusion method and dilution susceptibility tests. (ii) Determining the MIC of diacerein.

Techniques: Histopathology, Infection, Saline, Eye Drops, Staining, Negative Control, Positive Control